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, 32 (5), 423-430

Pathotype Classification of Plasmodiophora brassicae Isolates Using Clubroot-Resistant Cultivars of Chinese Cabbage

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Pathotype Classification of Plasmodiophora brassicae Isolates Using Clubroot-Resistant Cultivars of Chinese Cabbage

Hun Kim et al. Plant Pathol J.

Abstract

Clubroot disease caused by Plasmodiophora brassicae is one of the most serious diseases in Brassica crops worldwide. In this study, the pathotypes of 12 Korean P. brassicae field isolates were determined using various Chinese cabbage including 22 commercial cultivars from Korea, China, and Japan, and 15 inbred lines. All P. brassicae isolates exhibited the typical clubroot disease on non-clubroot resistant cultivar, indicating that the isolates were highly pathogenic. According to the reactions on the Williams' hosts, the 12 field isolates were initially classified into five races. However, when these isolates were inoculated onto clubroot-resistant (CR) cultivars of Chinese cabbage, several isolates led to different disease responses even though the isolates have been assigned to the same race by the Williams' host responses. Based on the pathogenicity results, the 12 field isolates were reclassified into four different groups: pathotype 1 (GN1, GN2, GS, JS, and HS), 2 (DJ and KS), 3 (HN1, PC, and YC), and 4 (HN2 and SS). In addition, the CR cultivars from Korea, China, and Japan exhibited distinguishable disease responses to the P. brassicae isolates, suggesting that the 22 cultivars used in this study, including the non-CR cultivars, are classified into four different host groups based on their disease resistance. Combining these findings, the four differential hosts of Chinese cabbage and four pathotype groups of P. brassicae might provide an efficient screening system for resistant cultivars and a new foundation of breeding strategies for CR Chinese cabbage.

Keywords: Chinese cabbage; breeding; clubroot; differential hosts; resistance.

Figures

Fig. 1
Fig. 1
Clubroot development on four representative cultivars of Chinese cabbage inoculated by a representative of each pathotype (GN1, pathotype 1; DJ, pathotype 2; YC, pathotype 3; and HN2, pathotype 4). The photos were taken five weeks after inoculation.

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References

    1. Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA. Study of physiologic specialization in Plasmodiophora brassicae: proposals for attempted rationalization through an international approach. Trans Br Mycol Soc. 1975;65:295–303. doi: 10.1016/S0007-1536(75)80013-1. - DOI
    1. Cho WD, Kim WG, Takahashi K. Occurrence of clubroot in cruciferous vegetable crops and races of the pathogen in Korea. Plant Pathol J. 2003;19:64–68. doi: 10.5423/PPJ.2003.19.1.064. - DOI
    1. Dixon GR. The occurrence and economic impact of Plasmodiophora brassicae and clubroot disease. J Plant Growth Regul. 2009;28:194–202. doi: 10.1007/s00344-009-9090-y. - DOI
    1. Hatakeyama K, Fujimura M, Ishida M, Suzuki T. New classification method for Plasmodiophora brassicae field isolates in Japan based on resistance of F1 cultivars of Chinese cabbage (Brassica rapa L.) to clubroot. Breed Sci. 2004;54:197–201. doi: 10.1270/jsbbs.54.197. - DOI
    1. Hirai M, Harada T, Kubo N, Tsukada M, Suwabe K, Matsumoto S. A novel locus for clubroot resistance in Brassica rapa and its linkage markers. Theor Appl Genet. 2004;108:639–643. doi: 10.1007/s00122-003-1475-x. - DOI - PubMed

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