A Two-Photon Excitation Based Fluorogenic Probe for Sialome Imaging in Living Systems

Lei Rong; Chi Zhang; Qi Lei; Si-Yong Qin; Jun Feng; Xian-Zheng Zhang

doi:10.1002/advs.201500211

A Two-Photon Excitation Based Fluorogenic Probe for Sialome Imaging in Living Systems

Adv Sci (Weinh). 2015 Sep 2;3(1):1500211. doi: 10.1002/advs.201500211. eCollection 2016 Jan.

Authors

Lei Rong¹, Chi Zhang², Qi Lei², Si-Yong Qin³, Jun Feng², Xian-Zheng Zhang²

Affiliations

¹ Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry Wuhan University Wuhan 430072 P.R. China; State Key Laboratory of Virology College of Life Sciences Wuhan University Wuhan 430072 P.R. China.
² Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry Wuhan University Wuhan 430072 P.R. China.
³ Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry Wuhan University Wuhan 430072 P.R. China; School of Chemistry and Materials Science South-Central University for Nationalities Wuhan 430074 P.R. China.

Abstract

Taking advantage of the fluorescence property of alkyne-modified naphthalimide, the two-photon excited fluorogenic probe Naph-yne is used for azide-tagged mannosyl glycoprotein imaging both at the cellular level and for vertebrate model organisms.

Keywords: click reaction; fluorescence labeling; glycoengineering; zebrafish.