An efficient two-photon fluorescent probe for monitoring mitochondrial singlet oxygen in tissues during photodynamic therapy

Chem Commun (Camb). 2016 Oct 11;52(83):12330-12333. doi: 10.1039/c6cc05880a.


A promising two-photon fluorescent probe MNAH for detecting 1O2 during the PDT process in mitochondria was proposed for the first time. MNAH was successfully applied for two-photon imaging of 1O2 in living cells and tissues during the PDT process with deep-tissue imaging depth. MNAH can be a powerful molecular tool for studying 1O2 generation in mitochondria during the PDT process.

MeSH terms

  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Mitochondria / metabolism*
  • Photochemotherapy*
  • Photons*
  • Singlet Oxygen / analysis*


  • Fluorescent Dyes
  • Singlet Oxygen