Serine is a new target residue for endogenous ADP-ribosylation on histones

Nat Chem Biol. 2016 Dec;12(12):998-1000. doi: 10.1038/nchembio.2180. Epub 2016 Oct 10.


ADP-ribosylation (ADPr) is a biologically and clinically important post-translational modification, but little is known about the amino acids it targets on cellular proteins. Here we present a proteomic approach for direct in vivo identification and quantification of ADPr sites on histones. We have identified 12 unique ADPr sites in human osteosarcoma cells and report serine ADPr as a new type of histone mark that responds to DNA damage.

MeSH terms

  • Adenosine Diphosphate / metabolism*
  • Cell Line, Tumor
  • DNA Damage
  • Histones / chemistry*
  • Histones / metabolism*
  • Humans
  • Protein Processing, Post-Translational*
  • Proteomics
  • Serine / metabolism*


  • Histones
  • Serine
  • Adenosine Diphosphate