Validation of Reference Genes for RT-qPCR Analysis in Burkholderia Pyrrocinia JK-SH007

J Microbiol Methods. 2017 Jan;132:95-98. doi: 10.1016/j.mimet.2016.10.004. Epub 2016 Oct 8.

Abstract

Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.

Keywords: Burkholderia pyrrocinia; Expression stability; RT-qPCR; Reference genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Burkholderia / classification
  • Burkholderia / genetics*
  • Burkholderia / isolation & purification*
  • Genes, Bacterial*
  • Real-Time Polymerase Chain Reaction*

Substances

  • Bacterial Proteins