Morphological, Genome and Gene Expression Changes in Newly Induced Autopolyploid Chrysanthemum lavandulifolium (Fisch. ex Trautv.) Makino

Int J Mol Sci. 2016 Oct 9;17(10):1690. doi: 10.3390/ijms17101690.

Abstract

Autopolyploidy is widespread in higher plants and plays an important role in the process of evolution. The present study successfully induced autotetraploidys from Chrysanthemum lavandulifolium by colchicine. The plant morphology, genomic, transcriptomic, and epigenetic changes between tetraploid and diploid plants were investigated. Ligulate flower, tubular flower and leaves of tetraploid plants were greater than those of the diploid plants. Compared with diploid plants, the genome changed as a consequence of polyploidization in tetraploid plants, namely, 1.1% lost fragments and 1.6% novel fragments occurred. In addition, DNA methylation increased after genome doubling in tetraploid plants. Among 485 common transcript-derived fragments (TDFs), which existed in tetraploid and diploid progenitors, 62 fragments were detected as differentially expressed TDFs, 6.8% of TDFs exhibited up-regulated gene expression in the tetraploid plants and 6.0% exhibited down-regulation. The present study provides a reference for further studying the autopolyploidization role in the evolution of C. lavandulifolium. In conclusion, the autopolyploid C. lavandulifolium showed a global change in morphology, genome and gene expression compared with corresponding diploid.

Keywords: AFLP (amplified fragment length polymorphism); Chrysanthemum lavandulifolium; MSAP (methylation sensitive amplified polymorphism); SRAP (sequence-related amplified polymorphism); morphology.

MeSH terms

  • Amplified Fragment Length Polymorphism Analysis
  • Chrysanthemum / anatomy & histology
  • Chrysanthemum / genetics*
  • DNA Methylation
  • DNA, Plant / genetics
  • DNA, Plant / metabolism
  • Epigenesis, Genetic
  • Gene Expression Regulation, Plant
  • Genome, Plant*
  • Tetraploidy

Substances

  • DNA, Plant