Mercury removal during growth of mercury tolerant and self-aggregating Yarrowia spp

Ganiyu Oladunjoye Oyetibo; Keisuke Miyauchi; Hitoshi Suzuki; Ginro Endo

doi:10.1186/s13568-016-0271-3

Mercury removal during growth of mercury tolerant and self-aggregating Yarrowia spp

AMB Express. 2016 Dec;6(1):99. doi: 10.1186/s13568-016-0271-3. Epub 2016 Oct 13.

Authors

Ganiyu Oladunjoye Oyetibo^{1

2}, Keisuke Miyauchi¹, Hitoshi Suzuki³, Ginro Endo⁴

Affiliations

¹ Department of Civil and Environmental Engineering, Faculty of Engineering, Tohoku-Gakuin University, 1-13-1 Chuo, Tagajo, Miyagi, 985-8537, Japan.
² Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Yaba, Lagos, Nigeria.
³ Department of Electronic Engineering, Faculty of Engineering, Tohoku-Gakuin University, 1-13-1 Chuo, Tagajo, Miyagi, 985-8537, Japan.
⁴ Department of Civil and Environmental Engineering, Faculty of Engineering, Tohoku-Gakuin University, 1-13-1 Chuo, Tagajo, Miyagi, 985-8537, Japan. gendo@mail.tohoku-gakuin.ac.jp.

Abstract

Ecotoxicological implications of mercury (Hg) pollution of hydrosphere require effective Hg-removal strategies as antidote to the environmental problems. Mercury-tolerant yeasts, Yarrowia spp. Idd1 and Idd2 strains, were studied for intracellular accumulation and extracellular micro-precipitation of Hg during growth stage of the yeast strains. In a liquid medium containing 870 (±23.6) µg of bioavailable Hg²⁺, 419.0 µg Hg²⁺ (approx.) was taken up by the wet biomasses of the yeast strains after 48 h post-inoculation. Large portion of the adsorbed Hg was found in cell wall (approx. 49-83 %) and spheroplast (approx. 62-89 %). Negligible quantities of Hg were present in the mitochondria (0.02-0.02 %), and appreciable amount of Hg was observed in nuclei and cell debris (15.2-65.3 %) as evidence of bioaccumulation. Extracellular polymeric substances (EPS) produced by the growing Yarrowia cells was a complex of protein, carbohydrates and other substances, immobilizing 43.8 (±0.7)-58.7 (±1.0) % of initial Hg in medium as micro-precipitates, while 10.13 ± 0.4-39.2 ± 4.3 % Hg content was volatilized. Transmission electron microscopy coupled with X-ray energy dispersive spectrophotometry confirmed the cellular removal of Hg and formation of EPS-Hg complex colloids in the surrounding bulk solution as micro-precipitates in form of extracellular Hg-nanoparticles. Hg mass balance in the bio-sequestration experiment revealed excellent Hg removal (>97 %) from the medium (containing ≤16 μg ml^-1 Hg²⁺) by the yeast strains via bioaccumulation, volatilization and micro-precipitation. The yeast strains are also effectively applicable in biological purification technology for Hg contaminated water because of their high self-aggregation activity and separatability from the aquatic environments. Graphical abstract Yarrowia species are oligotrophic marine yeasts that exhibited great potentials for mercuric ion remediation technologies, which are classified into four categories based on the process acting on the metal. These include immobilization through biosorption, compartmentation via bioaccumulation, separation from bulk solution via micro-precipitation upon EPS-Hg complex formation, and destruction that is a process to reduce the mercuric ion to metallic mercury.

Keywords: Bio-volatilization; Bioaccumulation; Extracellular polymeric substances; Mercury bio-removal; Micro-precipitation; Yarrowia.