Complete nucleotide sequence of a new variant of grapevine fanleaf virus from northeastern China

Jun Zhou; Xudong Fan; Yafeng Dong; Zunping Zhang; Fang Ren; Guojun Hu; Zhengnan Li

doi:10.1007/s00705-016-3101-7

Complete nucleotide sequence of a new variant of grapevine fanleaf virus from northeastern China

Arch Virol. 2017 Feb;162(2):577-579. doi: 10.1007/s00705-016-3101-7. Epub 2016 Oct 14.

Authors

Jun Zhou¹, Xudong Fan¹, Yafeng Dong², Zunping Zhang¹, Fang Ren¹, Guojun Hu¹, Zhengnan Li¹

Affiliations

¹ National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, 125100, Liaoning, China.
² National Center for Eliminating Viruses from Deciduous Fruit Tree, Research Institute of Pomology, Chinese Academy of Agriculture Sciences, Xingcheng, 125100, Liaoning, China. yfdong1234@163.com.

PMID: 27743254
DOI: 10.1007/s00705-016-3101-7

Abstract

The complete RNA1 and RNA2 sequences of a new grapevine fanleaf virus isolate (GFLV-SDHN) from northeastern China were determined. The two RNAs are 7,367 and 3,788 nucleotides (nt) in length, respectively, excluding the poly(A) tails. Compared to other GFLV isolates, GFLV-SDHN has a 22- to 24-nt insertion in the RNA1 5' untranslated region, and there was 19.1-20.1 % and 11.7 %-13.0 % sequence divergence in RNA1, and 15.5 %-20.5 % and 8.5-13.5 % in RNA2, at the nt and amino acid level, respectively. Phylogenetic analysis revealed that the origins of GFLV-SDHN are distinct from those of other GFLV isolates. One recombination event was identified in the 2A^HP region of RNA2 in GFLV-SDHN.

MeSH terms

Amino Acid Sequence
Base Sequence
China
Genome, Viral*
Nepovirus / classification
Nepovirus / genetics*
Nepovirus / isolation & purification
Phylogeny*
Plant Diseases / virology
Plant Leaves / virology
RNA, Viral / chemistry
RNA, Viral / genetics*
Recombination, Genetic
Vitis / virology*

Substances

RNA, Viral