Improvement of skin condition by oral administration of collagen hydrolysates in chronologically aged mice

J Sci Food Agric. 2017 Jul;97(9):2721-2726. doi: 10.1002/jsfa.8098. Epub 2016 Dec 9.


Background: Collagen hydrolysates (CHs) have been demonstrated to have positive effects on skin photoaging by topical application or oral ingestion. However, there has been little research on their influence on skin chronological aging. In this study, 9-month-old female ICR mice were given normal AIN-93M diets containing CHs (2.5, 5 and 10% w/w) from Nile tilapia scale.

Results: After 6 months, the collagen content and antioxidant enzyme (superoxide dismutase and glutathione peroxidase) activities increased significantly (P < 0.05), while the survival rate, viscera indices and contents of moisture, fat and non-collagenous protein in skin did not change (P > 0.05). The color, luster and quantity of hair were obviously ameliorated. Moreover, the structure of epidermis and dermis, the density and distribution of collagen fibers and the ratio of type I to type III collagen were improved in a dose-dependent manner as shown by histochemical staining.

Conclusion: Oral ingestion of CHs increased the collagen content and antioxidant enzyme activities and improved the appearance and structure of skin. These results suggest the potential of CHs as an anti-skin-aging ingredient in nutraceuticals or functional foods. © 2016 Society of Chemical Industry.

Keywords: chronological aging; collagen hydrolysates; histological structure; oxidative stress; skin condition; visual appearance.

MeSH terms

  • Administration, Oral
  • Animals
  • Antioxidants / metabolism
  • Collagen / chemistry
  • Collagen / metabolism*
  • Female
  • Fish Proteins / chemistry
  • Fish Proteins / metabolism*
  • Glutathione Peroxidase / metabolism
  • Humans
  • Mice
  • Mice, Inbred ICR
  • Protein Hydrolysates / chemistry
  • Protein Hydrolysates / metabolism*
  • Skin / enzymology
  • Skin / metabolism*
  • Skin / radiation effects
  • Skin Aging / radiation effects*
  • Superoxide Dismutase / metabolism
  • Tilapia
  • Ultraviolet Rays


  • Antioxidants
  • Fish Proteins
  • Protein Hydrolysates
  • Collagen
  • Glutathione Peroxidase
  • Superoxide Dismutase