Site specific protein O-glucosylation with bacterial toxins

Chem Commun (Camb). 2016 Oct 27;52(88):13024-13026. doi: 10.1039/c6cc06223g.


Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.

MeSH terms

  • Bacterial Proteins / metabolism*
  • Bacterial Toxins / metabolism*
  • Clostridioides difficile / growth & development
  • Clostridioides difficile / metabolism*
  • Glycosylation
  • Glycosyltransferases / metabolism*
  • Peptide Fragments / metabolism*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Bacterial Proteins
  • Bacterial Toxins
  • Peptide Fragments
  • Glycosyltransferases