The Large Subunit rDNA Sequence of Plasmodiophora brassicae Does not Contain Intra-species Polymorphism

Protist. 2016 Dec;167(6):544-554. doi: 10.1016/j.protis.2016.08.008. Epub 2016 Sep 9.


Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could allow to distinguish pathotypes without the need of time-consuming bioassays. However, isolates of P. brassicae from around the world analysed in this study do not show polymorphism in their LSU rDNA sequences. The previously described polymorphism most likely derived from soil inhabiting Cercozoa more specifically Neoheteromita-like glissomonads. Here we correct the LSU rDNA sequence of P. brassicae. By using FISH we demonstrate that our newly generated sequence belongs to the causal agent of clubroot disease.

Keywords: Clubroot; FISH (fluorescence in-situ hybridisation); LSU rDNA; pathotyping; phytomyxea; plasmodiophorids..

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brassica / parasitology
  • DNA, Protozoan / genetics*
  • DNA, Ribosomal / genetics*
  • In Situ Hybridization, Fluorescence
  • Plant Diseases / parasitology
  • Plasmodiophorida / genetics*
  • Polymorphism, Genetic*


  • DNA, Protozoan
  • DNA, Ribosomal