Evidence that chromium is an essential factor for biological activity of low-molecular-weight, chromium-binding substance

Biochem Biophys Res Commun. 1989 Aug 30;163(1):189-93. doi: 10.1016/0006-291x(89)92119-0.


Biologically active Low-molecular-weight, chromium-binding substance (LMCr) has been isolated from the liver of rabbits injected with potassium bicarbonate and cow's milk. This substance enhances glucose oxidation and lipogenesis from glucose in rat adipocytes [Yamamoto A. et al. (1987) Eur. J. Biochem. 165, 627-631; (1988) J. Nut. 118, 39-45]. LMCr was shown to lose its activity almost completely as an effectant of glucose metabolism when Cr was deleted under acidic conditions and separated from LMCr by EDTA-chelation and successive molecular-sieve chromatography. Reincorporation of Cr3+ into apo-LMCr resulted in 50-90% recovery of its original activity. These findings suggest that the biological activity of LMCr resides in Cr.

MeSH terms

  • Adipose Tissue / metabolism
  • Animals
  • Chromium / physiology*
  • Glucose / metabolism
  • In Vitro Techniques
  • Molecular Weight
  • Rats
  • Structure-Activity Relationship


  • Chromium
  • Glucose