Oligodeoxynucleotide Analogues of Circulating DNA Inhibit dsRNA-Induced Immune Response at the Early Stages of Signal Transduction Cascade in a Cell Type-Dependent Manner

Adv Exp Med Biol. 2016:924:105-107. doi: 10.1007/978-3-319-42044-8_20.

Abstract

Oligodeoxynucleotide (ODN) analogues of cell-surface-bound circulating DNA inhibit the dsRNA-induced production of pro-inflammatory interleukin 6, interferon beta and antibacterial peptide beta-defensin 2 not only in human gingival fibroblasts, but also in human primary endothelial and transformed cells (Hela and A431). ODN analogues do not effect dendritic cells activation by poly(I:C). The data obtained indicate that the early stages of the signal transduction cascade are violated by ODN analogues and the effects depend on the cell type.

Keywords: Circulating DNA; Double-stranded RNA; Innate immunity; Interferon; Interleukin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • DNA / blood
  • DNA / genetics*
  • HeLa Cells
  • Humans
  • Oligodeoxyribonucleotides / genetics*
  • RNA, Double-Stranded / genetics*
  • RNA, Double-Stranded / immunology
  • Signal Transduction / genetics*
  • Signal Transduction / immunology

Substances

  • Oligodeoxyribonucleotides
  • RNA, Double-Stranded
  • DNA