Purification of Circulating Cell-Free DNA from Plasma and Urine Using the Automated Large-Volume Extraction on the QIAsymphony® SP Instrument

Adv Exp Med Biol. 2016:924:179-185. doi: 10.1007/978-3-319-42044-8_33.

Abstract

Increasing sample numbers for screening and diagnostics using circulating cell-free DNA (ccfDNA) as analyte demands an automated solution for ccfDNA extraction. The efficiency of a new, automated, large volume ccfDNA extraction method was evaluated against a manual reference method. The new kit for automated ccfDNA extraction on the QIAsymphony showed a comparable yield of total ccfDNA from healthy donors as well as a comparable recovery of circulating cancer and fetal DNA. In conclusion, a new kit for automated ccfDNA extraction was established successfully.

Keywords: Automation; Cancer; Fetal; QIAGEN; QIAsymphony; ccfDNA.

MeSH terms

  • Automation / instrumentation*
  • Automation / methods*
  • DNA / blood
  • DNA / isolation & purification*
  • DNA / urine
  • Fetus / metabolism
  • Healthy Volunteers
  • Humans
  • Polymerase Chain Reaction / methods
  • Reagent Kits, Diagnostic*
  • Reproducibility of Results

Substances

  • Reagent Kits, Diagnostic
  • DNA