Assessment of anxiolytic effect of nerolidol in mice

Indian J Pharmacol. 2016 Jul-Aug;48(4):450-452. doi: 10.4103/0253-7613.186188.


Aim and objectives: The present study was to assess the anxiolytic effect of nerolidol in mice.

Materials and methods: The anxiolytic activity was examined using the elevated plus maze (EPM) and open field test (OFT), and motor coordination by rotarod test. Thirty Swiss albino mice were divided into five groups of six mice each. Group 1 received vehicle control (normal saline); Group 2 received diazepam (1 mg/kg); Groups 3, 4, and 5 received nerolidol 12.5, 25, and 50 mg/kg, respectively.

Results: Nerolidol (12.5, 25, and 50 mg/kg) significantly (P < 0.05) increased the time spent and a number of entries in open arm as compared to vehicle control in EPM test. In OFT, the nerolidol showed a significant (P < 0.05) increase in number of rearings and time spent in center and periphery, suggesting exploratory behavior of animals. Furthermore, nerolidol did not alter the fall down latency in rotarod test.

Conclusion: Our findings indicated that nerolidol exerts an anxiolytic effect without altering the motor coordination.

Keywords: Anxiety; diazepam; elevated plus maze; nerolidol; open field test.

MeSH terms

  • Animals
  • Anti-Anxiety Agents / pharmacology*
  • Behavior, Animal / drug effects*
  • Dose-Response Relationship, Drug
  • Exploratory Behavior / drug effects*
  • Male
  • Maze Learning / drug effects*
  • Mice, Inbred Strains
  • Psychomotor Performance / drug effects*
  • Rotarod Performance Test
  • Sesquiterpenes / pharmacology*


  • Anti-Anxiety Agents
  • Sesquiterpenes
  • nerolidol