Transcriptome response to elevated atmospheric CO2 concentration in the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)

Wenjing Wu; Zhiqiang Li; Shijun Zhang; Yunling Ke; Yahui Hou

doi:10.7717/peerj.2527

Transcriptome response to elevated atmospheric CO₂ concentration in the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae)

PeerJ. 2016 Oct 4:4:e2527. doi: 10.7717/peerj.2527. eCollection 2016.

Authors

Wenjing Wu¹, Zhiqiang Li¹, Shijun Zhang¹, Yunling Ke¹, Yahui Hou²

Affiliations

¹ Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources , Guangzhou , Guangdong , China.
² Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, Guangdong, China; College of Forestry, Northeast Forestry University, Harbin, Heilongjiang, China.

Abstract

Background: Carbon dioxide (CO₂) is a pervasive chemical stimulus that plays a critical role in insect life, eliciting behavioral and physiological responses across different species. High CO₂ concentration is a major feature of termite nests, which may be used as a cue for locating their nests. Termites also survive under an elevated CO₂ concentration. However, the mechanism by which elevated CO₂ concentration influences gene expression in termites is poorly understood.

Methods: To gain a better understanding of the molecular basis involved in the adaptation to CO₂ concentration, a transcriptome of Coptotermes formosanus Shiraki was constructed to assemble the reference genes, followed by comparative transcriptomic analyses across different CO₂ concentration (0.04%, 0.4%, 4% and 40%) treatments.

Results: (1) Based on a high throughput sequencing platform, we obtained approximately 20 GB of clean data and revealed 189,421 unigenes, with a mean length and an N50 length of 629 bp and 974 bp, respectively. (2) The transcriptomic response of C. formosanus to elevated CO₂ levels presented discontinuous changes. Comparative analysis of the transcriptomes revealed 2,936 genes regulated among 0.04%, 0.4%, 4% and 40% CO₂ concentration treatments, 909 genes derived from termites and 2,027 from gut symbionts. Genes derived from termites appears selectively activated under 4% CO₂ level. In 40% CO₂ level, most of the down-regulated genes were derived from symbionts. (3) Through similarity searches to data from other species, a number of protein sequences putatively involved in chemosensory reception were identified and characterized in C. formosanus, including odorant receptors, gustatory receptors, ionotropic receptors, odorant binding proteins, and chemosensory proteins.

Discussion: We found that most genes associated with carbohydrate metabolism, energy metabolism, and genetic information processing were regulated under different CO₂ concentrations. Results suggested that termites adapt to ∼4% CO₂ level and their gut symbionts may be killed under high CO₂ level. We anticipate that our findings provide insights into the transcriptome dynamics of CO₂ responses in termites and form the basis to gain a better understanding of regulatory networks.

Keywords: Carbon dioxide response; Chemosensory receptor; Coptotermes formosanus; Gene expression; Next generation sequencing data; Transcriptome.

Grants and funding

This research was supported by the National Natural Science Foundation of China (NSFC) (31172163), Funds for Environment Construction & Capacity Building of GDAS’ Research Platform (2016GDASPT-0107), and Science and Technology Planning Project of Guangzhou city, China (201510010036). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.