AtSWEET13 and AtSWEET14 regulate gibberellin-mediated physiological processes

Nat Commun. 2016 Oct 26;7:13245. doi: 10.1038/ncomms13245.

Abstract

Transmembrane transport of plant hormones is required for plant growth and development. Despite reports of a number of proteins that can transport the plant hormone gibberellin (GA), the mechanistic basis for GA transport and the identities of the transporters involved remain incomplete. Here, we provide evidence that Arabidopsis SWEET proteins, AtSWEET13 and AtSWEET14, which are members of a family that had previously been linked to sugar transport, are able to mediate cellular GA uptake when expressed in yeast and oocytes. A double sweet13 sweet14 mutant has a defect in anther dehiscence and this phenotype can be reversed by exogenous GA treatment. In addition, sweet13 sweet14 exhibits altered long distant transport of exogenously applied GA and altered responses to GA during germination and seedling stages. These results suggest that AtSWEET13 and AtSWEET14 may be involved in modulating GA response in Arabidopsis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Biological Transport / drug effects
  • Biological Transport / genetics
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Germination / genetics
  • Gibberellins / metabolism
  • Gibberellins / pharmacology*
  • Glucose / pharmacology
  • Hydrogen-Ion Concentration
  • Monosaccharide Transport Proteins / genetics
  • Monosaccharide Transport Proteins / metabolism*
  • Mutation
  • Plant Growth Regulators / metabolism
  • Plant Growth Regulators / pharmacology
  • Plant Physiological Phenomena / drug effects*
  • Plant Physiological Phenomena / genetics
  • Plants, Genetically Modified
  • Seedlings / genetics
  • Seedlings / growth & development
  • Seedlings / metabolism

Substances

  • Arabidopsis Proteins
  • AtSWEET13 protein, Arabidopsis
  • AtSWEET14 protein, Arabidopsis
  • Gibberellins
  • Monosaccharide Transport Proteins
  • Plant Growth Regulators
  • Glucose