A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.

Abstract

The advent and growth of synthetic biology has demonstrated its potential as a promising avenue of research to address many societal needs. However, plant synthetic biology efforts have been hampered by a dearth of DNA part libraries, versatile transformation vectors and efficient assembly strategies. Here, we describe a versatile system (named jStack) utilizing yeast homologous recombination to efficiently assemble DNA into plant transformation vectors. We demonstrate how this method can facilitate pathway engineering of molecules of pharmaceutical interest, production of potential biofuels and shuffling of disease-resistance traits between crop species. Our approach provides a powerful alternative to conventional strategies for stacking genes and traits to address many impending environmental and agricultural challenges.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biotechnology / methods*
  • Genetic Vectors / genetics*
  • Homologous Recombination*
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Plants / genetics*
  • Plants / metabolism
  • Plants, Genetically Modified
  • Reproducibility of Results
  • Saccharomyces cerevisiae / genetics*
  • Synthetic Biology / methods*