A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons

Chembiochem. 2016 Nov 17;17(22):2118-2122. doi: 10.1002/cbic.201600490. Epub 2016 Oct 26.

Abstract

Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2low cells from heterogeneous cell populations.

Keywords: ABC transporters; Abcg2; CDg13; cell recognition; fluorescent probes; neural stem/progenitor cells.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily G, Member 2 / antagonists & inhibitors
  • ATP Binding Cassette Transporter, Subfamily G, Member 2 / genetics
  • ATP Binding Cassette Transporter, Subfamily G, Member 2 / metabolism
  • Animals
  • Benzamides / chemistry*
  • Benzamides / metabolism
  • Biomarkers / metabolism
  • Brain / cytology
  • Brain / metabolism
  • Cell Differentiation
  • Cells, Cultured
  • Embryo, Mammalian / cytology
  • Fluorescent Dyes / chemistry*
  • Humans
  • Mice
  • Microscopy, Fluorescence
  • Neoplasm Proteins / antagonists & inhibitors
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / metabolism
  • Neural Stem Cells / chemistry
  • Neural Stem Cells / cytology
  • Neural Stem Cells / metabolism*
  • Neurons / cytology
  • Neurons / metabolism*
  • Propionates / chemistry
  • Propionates / metabolism
  • Quinolines / chemistry
  • Quinolines / metabolism
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Xanthenes / chemistry*
  • Xanthenes / metabolism

Substances

  • ABCG2 protein, human
  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • Benzamides
  • Biomarkers
  • CDg13 compound
  • Fluorescent Dyes
  • Neoplasm Proteins
  • Propionates
  • Quinolines
  • RNA, Small Interfering
  • Xanthenes
  • verlukast