Sensitivity of C6 Glioma Cells Carrying the Human Poliovirus Receptor to Oncolytic Polioviruses

A O Sosnovtseva; A V Lipatova; N F Grinenko; V P Baklaushev; P M Chumakov; V P Chekhonin

doi:10.1007/s10517-016-3520-1

Sensitivity of C6 Glioma Cells Carrying the Human Poliovirus Receptor to Oncolytic Polioviruses

Bull Exp Biol Med. 2016 Oct;161(6):821-825. doi: 10.1007/s10517-016-3520-1. Epub 2016 Oct 26.

Authors

A O Sosnovtseva^{1

2}, A V Lipatova³, N F Grinenko⁴, V P Baklaushev⁵, P M Chumakov³, V P Chekhonin^{5

4}

Affiliations

¹ Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia. aososnovtceva@gmail.com.
² Laboratory of Cell Proliferation, V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. aososnovtceva@gmail.com.
³ Laboratory of Cell Proliferation, V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
⁴ Department of Basic and Applied Neurobiology, V. P. Serbsky Federal Medical Research Centre of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, Russia.
⁵ Department of Medical Nanobiotechnologies, Medical and Biological Faculty, N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.

PMID: 27783287
DOI: 10.1007/s10517-016-3520-1

Abstract

A humanized line of rat C6 glioma cells expressing human poliovirus receptor was obtained and tested for the sensitivity to oncolytic effects of vaccine strains of type 1, 2, and 3 polioviruses. Presentation of the poliovirus receptor on the surface of C6 glioma cells was shown to be a necessary condition for the interaction of cells with polioviruses, but insufficient for complete poliovirus oncolysis.

Keywords: malignant gliomas; oncolytic viruses; poliovirus; poliovirus receptor; virotherapy.

MeSH terms

Animals
Cell Line, Tumor
Cell Survival
Gene Expression
Genetic Vectors / chemistry
Genetic Vectors / metabolism
Humans
Lentivirus / genetics
Lentivirus / metabolism
Luminescent Proteins / genetics
Luminescent Proteins / metabolism
Neuroglia / metabolism
Neuroglia / pathology
Neuroglia / virology*
Oncolytic Virotherapy / methods
Oncolytic Viruses / physiology*
Poliovirus / physiology*
Protein Binding
Rats
Receptors, Virus / genetics*
Receptors, Virus / metabolism
Recombinant Fusion Proteins / genetics*
Recombinant Fusion Proteins / metabolism
Transduction, Genetic
Transgenes
Viral Load / physiology
Virus Replication / physiology

Substances

Luminescent Proteins
Receptors, Virus
Recombinant Fusion Proteins
blue fluorescent protein, Aequorea victoria
poliovirus receptor