doi: 10.1007/978-1-4939-6448-2_2.
Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells
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- PMID: 27787840
- DOI: 10.1007/978-1-4939-6448-2_2
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Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells
Methods Mol Biol.
2017.
Abstract
Recombinant proteins are widely used in biomedical sciences, and in pharmaceutical research. Through genetic recombination, specific DNA sequences are engineered and inserted into a biological host. Once inside the host, the DNA is transcribed and translated into the target protein and is ready to be purified. Here, we describe the transfection, purification, and visualization of Fc-tagged SEMA4D (semaphorin 4D) recombinant protein.
Keywords: Affinity chromatography; Lipofectamine; Lipofection; Protein affinity purification; Recombinant protein; SDS-PAGE; Silver stain.
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