Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells

Xiao Huang; Qirui Hu; Yifan Lai; Demosthenes P Morales; Dennis O Clegg; Norbert O Reich

doi:10.1002/adma.201603318

Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells

Adv Mater. 2016 Dec;28(48):10732-10737. doi: 10.1002/adma.201603318. Epub 2016 Oct 27.

Authors

Xiao Huang¹, Qirui Hu², Yifan Lai¹, Demosthenes P Morales¹, Dennis O Clegg², Norbert O Reich¹

Affiliations

¹ Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA.
² Center for Stem Cell Biology and Engineering, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, 93106, USA.

PMID: 27787919
DOI: 10.1002/adma.201603318

Abstract

A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.

Keywords: 3D cultures; RNAi; human embryonic stem cells; near-infrared light; spatial control.

MeSH terms

Cell Culture Techniques*
Down-Regulation
Gold
Human Embryonic Stem Cells / cytology
Human Embryonic Stem Cells / metabolism*
Human Embryonic Stem Cells / radiation effects*
Humans
Infrared Rays
Microscopy, Fluorescence, Multiphoton
Nanoshells
RNA Interference / radiation effects*
RNA, Small Interfering / genetics*
RNA, Small Interfering / radiation effects*

Substances

RNA, Small Interfering
Gold