Episomal plasmid-based generation of an iPSC line from a 79-year-old individual carrying the APOE4/4 genotype: i11001

Stem Cell Res. 2016 Nov;17(3):544-546. doi: 10.1016/j.scr.2016.09.023. Epub 2016 Sep 23.

Abstract

In this study, lymphoblastoid cells derived from a 79-year old individual with a history of progressive presenile dementia, were used to generate iPS cells, employing episomal plasmids expressing OCT4, SOX2, KLF4, LIN28, L-MYC and a p53 shRNA. The individual was homozygous for the APOE4 allele. The resulting iPS cells had a normal karyotype, retained the APOE4/4 genotype, expressed pluripotency markers, were free of genomically integrated plasmids, and could be differentiated into cell type representatives from the three germ layers in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Apolipoprotein E4 / genetics*
  • B-Lymphocytes / cytology
  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming
  • Dementia / genetics
  • Dementia / pathology*
  • Embryoid Bodies / cytology
  • Embryoid Bodies / metabolism
  • Genotype
  • Homozygote
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Microscopy, Fluorescence
  • Plasmids / genetics
  • Plasmids / metabolism
  • Polymorphism, Single Nucleotide
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Tumor Suppressor Protein p53 / antagonists & inhibitors
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism

Substances

  • Apolipoprotein E4
  • RNA, Small Interfering
  • TP53 protein, human
  • Transcription Factors
  • Tumor Suppressor Protein p53