Interleukin 10 gene single nucleotide polymorphisms in Polish patients with chronic hepatitis C: Analysis of association with severity of disease and treatment outcome

Hum Immunol. 2017 Feb;78(2):192-200. doi: 10.1016/j.humimm.2016.10.015. Epub 2016 Oct 26.

Abstract

It is suggested that interleukin 10 (IL-10), as a modulator of immune response, is likely to influence the elimination of hepatitis C virus (HCV), the progression of chronic hepatitis C (CHC) and the response to interferon-based therapy in CHC patients. The aim of the study was to analyze the association of single nucleotide polymorphisms (SNPs) of IL-10 gene with severity of liver disease (degree of inflammation and stage of fibrosis) and outcome of pegylated interferon alpha and ribavirin combined therapy (sustained virological response (SVR) and relapse) in 196 Polish CHC patients infected with HCV genotype 1. The analysis included IL-10 promoter SNPs: -1082(A/G) rs1800896, -819(C/T) rs1800871, -592(C/A) rs1800872 and SNP in the 3' UTR of IL-10 gene: +4529(A/G) rs3024498. Genotyping was performed using PCR-RFLP and HRM analysis. It was demonstrated that the -592C allele is associated with mild hepatic inflammation. Moreover, it was found that the -819C allele might be associated with SVR and that the ACCA haplotype and intermediate IL-10 producer ACC haplotype are associated with SVR and non-relapse. It can be concluded that IL-10 SNPs are associated with severity of disease and response to therapy and may be considered as potential prognostic and predictive markers in CHC.

Keywords: Chronic hepatitis C; Hepatitis C virus; Interleukin 10; Single nucleotide polymorphism.

MeSH terms

  • Adult
  • Disease Progression
  • Drug Therapy, Combination
  • Female
  • Fibrosis
  • Gene Frequency
  • Genotype
  • Hepatitis C, Chronic / genetics*
  • Hepatitis C, Chronic / therapy
  • Humans
  • Inflammation / genetics*
  • Interferon-alpha / therapeutic use
  • Interleukin-10 / genetics*
  • Liver / immunology
  • Liver / pathology*
  • Male
  • Middle Aged
  • Poland
  • Polyethylene Glycols / therapeutic use
  • Polymorphism, Single Nucleotide*
  • Recombinant Proteins / therapeutic use
  • Ribavirin / therapeutic use
  • Treatment Outcome
  • Young Adult

Substances

  • Interferon-alpha
  • Recombinant Proteins
  • Interleukin-10
  • Polyethylene Glycols
  • Ribavirin
  • peginterferon alfa-2a