Pulsed Electromagnetic Field with Temozolomide Can Elicit an Epigenetic Pro-apoptotic Effect on Glioblastoma T98G Cells

Anticancer Res. 2016 Nov;36(11):5821-5826. doi: 10.21873/anticanres.11166.

Abstract

Treatment with pulsed electromagnetic fields (PEMFs) is emerging as an interesting therapeutic option for patients with cancer. The literature has demonstrated that low-frequency/low-energy electromagnetic fields do not cause predictable effects on DNA; however, they can epigenetically act on gene expression. The aim of the present work was to study a possible epigenetic effect of a PEMF, mediated by miRNAs, on a human glioblastoma cell line (T98G). We tested a PEMF (maximum magnetic induction, 2 mT; frequency, 75 Hz) that has been demonstrated to induce autophagy in glioblastoma cells. In particular, we studied the effect of PEMF on the expression of genes involved in cancer progression and a promising synergistic effect with temozolomide, a frequently used drug to treat glioblastoma multiforme. We found that electromagnetic stimulation in combination with temozolomide can elicit an epigenetic pro-apoptotic effect in the chemo- and radioresistant T98G glioblastoma cell line.

Keywords: Glioblastoma multiforme; epigenetic pro-apoptotic effect; pulsed electromagnetic stimulation; temozolomide.

MeSH terms

  • Antineoplastic Agents, Alkylating / pharmacology*
  • Apoptosis / drug effects*
  • Apoptosis / genetics
  • Bioreactors
  • Cell Proliferation / drug effects
  • Dacarbazine / analogs & derivatives*
  • Dacarbazine / pharmacology
  • Electromagnetic Fields*
  • Epigenesis, Genetic / drug effects*
  • Glioblastoma / pathology*
  • Humans
  • MicroRNAs / genetics
  • Temozolomide

Substances

  • Antineoplastic Agents, Alkylating
  • MicroRNAs
  • Dacarbazine
  • Temozolomide