In Vitro Activities of Novel Azole Compounds ATTAF-1 and ATTAF-2 against Fluconazole-Susceptible and -Resistant Isolates of Candida Species

Hamed Fakhim; Saeed Emami; Afsane Vaezi; Seyedeh Mahdieh Hashemi; Leila Faeli; Kambiz Diba; Eric Dannaoui; Hamid Badali

doi:10.1128/AAC.01106-16

In Vitro Activities of Novel Azole Compounds ATTAF-1 and ATTAF-2 against Fluconazole-Susceptible and -Resistant Isolates of Candida Species

Antimicrob Agents Chemother. 2016 Dec 27;61(1):e01106-16. doi: 10.1128/AAC.01106-16. Print 2017 Jan.

Authors

Hamed Fakhim^{1

2}, Saeed Emami³, Afsane Vaezi^{1

2}, Seyedeh Mahdieh Hashemi³, Leila Faeli^{1

2}, Kambiz Diba⁴, Eric Dannaoui⁵, Hamid Badali^{6

7}

Affiliations

¹ Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
² Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
³ Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
⁴ Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
⁵ Université Paris-Descartes, Faculté de Médecine, APHP, Hôpital Européen Georges Pompidou, Unité de Parasitologie-Mycologie, Service de Microbiologie, Paris, France.
⁶ Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran badalii@yahoo.com.
⁷ Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Abstract

The in vitro activities of two novel azole compounds (aryl-1,2,4-triazol-3-ylthio analogues of fluconazole [ATTAFs]) and five comparator antifungal agents against 52 clinical Candida isolates from 5 different species were determined. The novel azole compounds had the lowest geometric mean MICs, followed by fluconazole. Moreover, combinations of these compounds with fluconazole exhibited synergistic effects against fluconazole-susceptible (22 of 23 isolates), fluconazole-susceptible dose-dependent (10 of 13 isolates), and fluconazole-resistant (1 of 16 isolates) Candida isolates.

Keywords: Candida species; In vitro susceptibility; triazole derivatives.

MeSH terms

Antifungal Agents / pharmacology*
Azoles / pharmacology*
Candida / drug effects
Drug Resistance, Fungal / genetics
Fluconazole / pharmacology*
Microbial Sensitivity Tests
Triazoles / pharmacology

Substances

Antifungal Agents
Azoles
Triazoles
Fluconazole