Protein and Chromosome Analysis in Mammalian Meiocytes

Methods Mol Biol. 2017;1515:97-111. doi: 10.1007/978-1-4939-6545-8_6.

Abstract

Meiosis is a highly specialized cell division that facilitates the production of haploid gametes from diploid mother cells. It is characterized by unique chromatin structures and chromatin associated protein complexes. The analysis of these structures and complexes has greatly benefited from and relied on the visualization of meiotic proteins in diverse preparations of meiocytes. In this chapter we summarize methods that can be used for the characterization of the behavior and localization of meiotic proteins in mammalian meiocytes.

Keywords: Chromosomes; Germ cell analyses; Meiosis; Oogenesis; Spermatogenesis.

MeSH terms

  • Animals
  • Chromatin / genetics*
  • Chromosomes / genetics*
  • Germ Cells / growth & development
  • Mammals / genetics
  • Meiosis / genetics*
  • Molecular Biology / methods*
  • Oogenesis / genetics
  • Spermatogenesis / genetics

Substances

  • Chromatin