Single-cell sequencing of the small-RNA transcriptome

Nat Biotechnol. 2016 Dec;34(12):1264-1266. doi: 10.1038/nbt.3701. Epub 2016 Oct 31.


Little is known about the heterogeneity of small-RNA expression as small-RNA profiling has so far required large numbers of cells. Here we present a single-cell method for small-RNA sequencing and apply it to naive and primed human embryonic stem cells and cancer cells. Analysis of microRNAs and fragments of tRNAs and small nucleolar RNAs (snoRNAs) reveals the potential of microRNAs as markers for different cell types and states.

MeSH terms

  • Cell Line, Tumor
  • Cells, Cultured
  • High-Throughput Nucleotide Sequencing / methods*
  • Human Embryonic Stem Cells / physiology*
  • Humans
  • MicroRNAs / genetics*
  • Neoplasms, Experimental / genetics*
  • Sequence Analysis, RNA / methods*
  • Transcriptome / genetics*


  • MicroRNAs