Fluorescent indicators for simultaneous reporting of all four cell cycle phases

Nat Methods. 2016 Dec;13(12):993-996. doi: 10.1038/nmeth.4045. Epub 2016 Oct 31.

Abstract

A robust method for simultaneous visualization of all four cell cycle phases in living cells is highly desirable. We developed an intensiometric reporter of the transition from S to G2 phase and engineered a far-red fluorescent protein, mMaroon1, to visualize chromatin condensation in mitosis. We combined these new reporters with the previously described Fucci system to create Fucci4, a set of four orthogonal fluorescent indicators that together resolve all cell cycle phases.

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Cycle / physiology*
  • Chromatin / metabolism
  • G2 Phase / physiology
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Luminescent Proteins / chemistry*
  • Luminescent Proteins / genetics
  • Mice
  • Mitosis
  • Models, Molecular
  • Molecular Imaging / methods*
  • NIH 3T3 Cells
  • Recombinant Fusion Proteins / chemistry*
  • Recombinant Fusion Proteins / genetics
  • S Phase / physiology
  • Time-Lapse Imaging / methods*

Substances

  • Chromatin
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • red fluorescent protein