Incidence of Keratinophilic Fungi from the Selected Soils of Kaziranga National Park, Assam (India)

Mycopathologia. 2017 Apr;182(3-4):371-377. doi: 10.1007/s11046-016-0083-7. Epub 2016 Oct 31.

Abstract

Seventy-eight soil samples were collected from the various locations in the vicinity of Kaziranga National Park (Assam), India, during April to October 2009 and screened for the presence of keratinophilic fungi using the hair baiting techniques for isolation. Thirty-nine isolates were recovered and identified by recognition of their macro- and micromorphological features. Their identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/GenBank data and compared with deposited sequences for identification purpose. Eleven species related to seven genera were recorded viz. Aphanoascus durus (1.28%), Arthroderma tuberculatum (3.84%), Arthroderma corniculatum (1.28%), Chrysosporium indicum (16.66%), C. tropicum (3.84%), Ctenomyces serratus (5.12%), Keratinophyton punsolae (1.28%), Microsporum appendiculatum (1.28%), Microsporum gypseum complex (11.53%), Trichophyton mentagrophytes (11.28%) and T. terrestre (2.56%).

Keywords: Assam; India; Kaziranga National Park; Keratinophilic fungi; Soil fungi.

MeSH terms

  • Cluster Analysis
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Fungi / classification
  • Fungi / genetics
  • Fungi / isolation & purification*
  • Fungi / metabolism*
  • Hair / microbiology
  • Incidence
  • India
  • Keratins / metabolism*
  • Microbiological Techniques
  • Microscopy
  • Parks, Recreational
  • Phylogeny
  • RNA, Ribosomal, 5.8S
  • Sequence Analysis, DNA
  • Soil Microbiology*

Substances

  • DNA, Fungal
  • DNA, Ribosomal
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 5.8S
  • Keratins