Collagen Extract from Marine Finfish Scales as a Potential Mosquito Larvicide

Protein J. 2016 Dec;35(6):391-400. doi: 10.1007/s10930-016-9685-7.

Abstract

Collagen is a peptide being utilized in medical, health care, nutrient and decorative industry. Marine fish scales are one of the good sources of collagen, which is extracted using the advanced enzymatic digestion method. Scales of Sardinella longiceps (Oil Sardine) have a high proportion of collagen. This product is well absorbed with broad adaptive values that encourage the inclusion of nutriments. In this paper, we have performed the isolation and characterization of collagen from S. longiceps fish scales. The unnecessary proteins on the surface of fish scales was removed by demineralization process. The fish scale collagen was extracted in two different methods: acid (acetic acid) and enzymatic (pepsin) technique. The molecular mass of the extracted collagen was determined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The absorption spectra of the extracted collagen was measured to estimate its amino acid (tyrosine) content. Fourier transform infrared (FTIR) spectrum showed the existence of bands corresponding to the collagen extracted from S. longiceps fish scale and the crystallinity of extracted collagen was obtained using X-ray diffraction (XRD) analysis. The morphological micrograph was also analyzed by scanning electron microscope (SEM). The anti-larval effect of the collagen extract was determined using mosquito larvae of Aedes aegypti (Ae. aegypti) and the activity was statistically significant.

Keywords: Acid and pepsin soluble collagen; Aedes aegypti; Fish collagen; Sardinella longiceps.

MeSH terms

  • Acetic Acid / chemistry
  • Aedes / drug effects*
  • Aedes / growth & development
  • Animals
  • Collagen / isolation & purification*
  • Collagen / ultrastructure
  • Dose-Response Relationship, Drug
  • Fishes
  • Insecticides / chemistry
  • Insecticides / isolation & purification*
  • Larva / drug effects*
  • Larva / growth & development
  • Liquid-Liquid Extraction / methods*
  • Microscopy, Electron, Scanning
  • Pepsin A / chemistry
  • Skin / chemistry*
  • Solvents / chemistry

Substances

  • Insecticides
  • Solvents
  • Collagen
  • Pepsin A
  • Acetic Acid