Control of bacterial nitrate assimilation by stabilization of G-quadruplex DNA

Chem Commun (Camb). 2016 Nov 28;52(92):13511-13514. doi: 10.1039/c6cc06057a. Epub 2016 Nov 2.


Here we present a chemical-biology study in the model soil bacterium Paracoccus denitrificans, where we show ligand-specific control of nitrate assimilation. Stabilization of a G-quadruplex in the promoter region of the nas genes, encoding the assimilatory nitrate/nitrite reductase system, is achieved using known quadruplex ligands and results in attenuation of gene transcription.

MeSH terms

  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism*
  • G-Quadruplexes*
  • Ligands
  • Nitrates / chemistry
  • Nitrates / metabolism*
  • Paracoccus denitrificans / genetics
  • Paracoccus denitrificans / metabolism*


  • DNA, Bacterial
  • Ligands
  • Nitrates