MRZH reaction increases sensitivity for intrathecal IgG synthesis in IgG Oligoclonal band negative Multiple Sclerosis patients

J Neuroimmunol. 2016 Nov 15;300:30-35. doi: 10.1016/j.jneuroim.2016.10.001. Epub 2016 Oct 5.


Given the low detection rates of CSF IgG-Oligoclonal bands (IgG-OCB) in non-European Multiple Sclerosis (MS) patients and higher specificity of the MRZH-reaction, we evaluated whether associating MRZH-reaction to CSF IgG-OCB detection improved investigation of suspected MS. Paired CSF and sera were analyzed for IgG-OCB and polyspecific viral antibodies. IgG-OCB were detected in 72% of MS patients and an MRZH-reaction in 67%. Combining IgG-OCB and MRZH raised detection of IgG abnormalities to 97% of studied MS patients. Detection of IgG-OCB and/or ≥2 MRZH antibodies showed sensitivity of 88% and specificity of 92% for MS, versus 72% and 96% for IgG-OCB alone.

Keywords: Brazil; Cerebrospinal Fluid; Intrathecal synthesis; Multiple Sclerosis; Oligoclonal bands; Polyspecific antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Viral / cerebrospinal fluid*
  • Biomarkers / cerebrospinal fluid
  • Female
  • Herpesvirus 3, Human / metabolism
  • Humans
  • Immunoglobulin G / biosynthesis*
  • Immunoglobulin G / cerebrospinal fluid*
  • Male
  • Measles virus / metabolism
  • Middle Aged
  • Multiple Sclerosis / cerebrospinal fluid*
  • Multiple Sclerosis / diagnosis*
  • Oligoclonal Bands / cerebrospinal fluid*
  • Rubella virus / metabolism
  • Simplexvirus / metabolism
  • Young Adult


  • Antibodies, Viral
  • Biomarkers
  • Immunoglobulin G
  • Oligoclonal Bands