HJURP interaction with the condensin II complex during G1 promotes CENP-A deposition

Mol Biol Cell. 2017 Jan 1;28(1):54-64. doi: 10.1091/mbc.E15-12-0843. Epub 2016 Nov 2.


Centromeric chromatin is required for kinetochore assembly during mitosis and accurate chromosome segregation. A unique nucleosome containing the histone H3-specific variant CENP-A is the defining feature of centromeric chromatin. In humans, CENP-A nucleosome deposition occurs in early G1 just after mitotic exit at the time when the CENP-A deposition machinery localizes to centromeres. The mechanism by which CENP-A is deposited onto an existing, condensed chromatin template is not understood. Here we identify the selective association of the CENP-A chaperone HJURP with the condensin II complex and not condensin I. We show CAPH2 is present at centromeres during early G1 at the time when CENP-A deposition is occurring. CAPH2 localization to early G1 centromeres is dependent on HJURP. The CENP-A chaperone and assembly factor HJURP induces decondensation of a noncentromeric LacO array, and this decondensation is modulated by the condensin II complex. We show that condensin II function at the centromere is required for new CENP-A deposition in human cells. These data demonstrate that HJURP selectively recruits the condensin II chromatin-remodeling complex to facilitate CENP-A deposition in human cells.

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Autoantigens / metabolism*
  • Centromere / metabolism*
  • Centromere Protein A
  • Chromatin / metabolism
  • Chromatin Assembly and Disassembly / physiology
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Chromosome Segregation / physiology
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • DNA-Binding Proteins / physiology
  • G1 Phase
  • Histones / metabolism
  • Humans
  • Mitosis
  • Multiprotein Complexes / metabolism
  • Nucleosomes
  • Protein Binding / physiology
  • Serine Endopeptidases / metabolism
  • Serine Endopeptidases / physiology
  • Succinate Dehydrogenase / metabolism


  • Autoantigens
  • CENPA protein, human
  • Centromere Protein A
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • HJURP protein, human
  • Histones
  • Multiprotein Complexes
  • Nucleosomes
  • SDHD protein, human
  • condensin complexes
  • Succinate Dehydrogenase
  • PRSS27 protein, human
  • Serine Endopeptidases
  • Adenosine Triphosphatases