Multidimensional chemical control of CRISPR-Cas9

Nat Chem Biol. 2017 Jan;13(1):9-11. doi: 10.1038/nchembio.2224. Epub 2016 Oct 31.


Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred such multidimensional controls to diverse Cas9 systems by leveraging small-molecule-regulated protein degron domains. Application of our strategy to both Cas9-mediated genome editing and transcriptional activities opens new avenues for systematic genome interrogation.

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Cells, Cultured
  • Gene Editing / methods*
  • HEK293 Cells
  • Humans
  • INDEL Mutation / genetics
  • Real-Time Polymerase Chain Reaction
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*
  • Transcription, Genetic / genetics


  • Transcription Factors