IncFII Conjugative Plasmid-Mediated Transmission of blaNDM-1 Elements among Animal-Borne Escherichia coli Strains

Dachuan Lin; Miaomiao Xie; Ruichao Li; Kaichao Chen; Edward Wai-Chi Chan; Sheng Chen

doi:10.1128/AAC.02285-16

IncFII Conjugative Plasmid-Mediated Transmission of blaNDM-1 Elements among Animal-Borne Escherichia coli Strains

Antimicrob Agents Chemother. 2016 Dec 27;61(1):e02285-16. doi: 10.1128/AAC.02285-16. Print 2017 Jan.

Authors

Dachuan Lin^{1

2}, Miaomiao Xie^{1

2}, Ruichao Li^{1

2}, Kaichao Chen^{1

2}, Edward Wai-Chi Chan², Sheng Chen^{3

2}

Affiliations

¹ Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People's Republic of China.
² The State Key Laboratory of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.
³ Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People's Republic of China sheng.chen@polyu.edu.hk.

Abstract

This study aims to investigate the prevalence and transmission dynamics of the bla_NDM-1 gene in animal Escherichia coli strains. Two IncFII bla_NDM-1-encoding plasmids with only minor structural variation in the MDR region, pHNEC46-NDM and pHNEC55-NDM, were found to be responsible for the transmission of bla_NDM-1 in these strains. The bla_NDM-1 gene can be incorporated into plasmids and stably inherited in animal-borne E. coli strains that can be maintained in animal gut microflora even without carbapenem selection pressure.

Keywords: Escherichia coli; IncFII; animal; blaNDM-1; plasmid sequence; transposons.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Carbapenems / pharmacology
China / epidemiology
Drug Resistance, Multiple, Bacterial
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli / growth & development
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology
Escherichia coli Infections / microbiology
Escherichia coli Infections / transmission
Escherichia coli Infections / veterinary*
Feces / microbiology
Gene Expression
Plasmids / chemistry*
Plasmids / metabolism
Prevalence
Swine
Swine Diseases / epidemiology*
Swine Diseases / microbiology
Swine Diseases / transmission
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
Carbapenems
beta-Lactamases
beta-lactamase NDM-1