Lack of CaBP1/Caldendrin or CaBP2 Leads to Altered Ganglion Cell Responses

eNeuro. 2016 Oct 28;3(5):ENEURO.0099-16.2016. doi: 10.1523/ENEURO.0099-16.2016. eCollection 2016 Sep-Oct.

Abstract

Calcium-binding proteins (CaBPs) form a subfamily of calmodulin-like proteins that were cloned from the retina. CaBP4 and CaBP5 have been shown to be important for normal visual function. Although CaBP1/caldendrin and CaBP2 have been shown to modulate various targets in vitro, it is not known whether they contribute to the transmission of light responses through the retina. Therefore, we generated mice that lack CaBP2 or CaBP1/caldendrin (Cabp2-/- and Cabp1-/- ) to test whether these CaBPs are essential for normal retinal function. By immunohistochemistry, the overall morphology of Cabp1-/- and Cabp2-/- retinas and the number of synaptic ribbons appear normal; transmission electron microscopy shows normal tethered ribbon synapses and synaptic vesicles as in wild-type retinas. However, whole-cell patch clamp recordings showed that light responses of retinal ganglion cells of Cabp2-/- and Cabp1-/- mice differ in amplitude and kinetics from those of wild-type mice. We conclude that CaBP1/caldendrin and CaBP2 are not required for normal gross retinal and synapse morphology but are necessary for the proper transmission of light responses through the retina; like other CaBPs, CaBP1/caldendrin and CaBP2 likely act by modulating presynaptic Ca2+-dependent signaling mechanisms.

Keywords: bipolar cells; calcium-binding proteins; ganglion cells; knockout mice; light response; retina.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Animals
  • Calcium-Binding Proteins / deficiency*
  • Calcium-Binding Proteins / genetics
  • Immunohistochemistry
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Electron
  • Patch-Clamp Techniques
  • Photic Stimulation
  • Retinal Bipolar Cells / metabolism
  • Retinal Bipolar Cells / ultrastructure
  • Retinal Ganglion Cells / metabolism*
  • Retinal Ganglion Cells / ultrastructure
  • Synapses / metabolism
  • Synapses / ultrastructure
  • Tissue Culture Techniques
  • Vision, Ocular / physiology

Substances

  • Calcium-Binding Proteins
  • Ca-binding protein 2
  • Ca2+-binding protein-1