Proteomics profiling of interactome dynamics by colocalisation analysis (COLA)

Mol Biosyst. 2016 Dec 20;13(1):92-105. doi: 10.1039/c6mb00701e.


Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein-protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.

MeSH terms

  • Cell Fractionation
  • Cell Line
  • Chromatography, Liquid
  • Cluster Analysis
  • Humans
  • Intracellular Space / metabolism
  • Mass Spectrometry
  • Protein Binding
  • Protein Interaction Mapping / methods*
  • Protein Interaction Maps*
  • Protein Transport
  • Proteome*
  • Proteomics* / methods
  • Sensitivity and Specificity
  • Subcellular Fractions


  • Proteome