Probing the function of the two holin-like proteins of bacteriophage SPP1

Virology. 2017 Jan:500:184-189. doi: 10.1016/j.virol.2016.10.030. Epub 2016 Nov 5.

Abstract

Double-stranded DNA bacteriophages employ holin and endolysin functions to lyse host bacteria after virus multiplication. Holins oligomerize in the cytoplasmic membrane and trigger to form holes that cause cell death. For most systems these holes are also required for endolysin release to the cell wall, where it cleaves the peptidoglycan network. Orfs 25 and 26 of Bacillus subtilis phage SPP1 were predicted to encode the endolysin and holin functions, respectively. However, the product of the upstream orf 24.1 exhibits also holin features. We show that production of gp24.1 or gp26 in B. subtilis causes no major impact on cell growth, despite their ability to insert in the cytoplasmic membrane. Instant growth cessation and cell death is observed only upon co-production of the two holin-like proteins. Surprisingly, a constitutive promoter was identified within orf 24.1, which we propose to correspond to the previously described SPP1 early promoter PE5.

Keywords: B. subtilis; Bacteriophage; Endolysin; Holin; Lysis; Promoter; SPP1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacillus subtilis / growth & development
  • Bacillus subtilis / virology*
  • Bacteriophages / genetics
  • Bacteriophages / metabolism*
  • Endopeptidases / genetics
  • Endopeptidases / metabolism*
  • Gene Expression Regulation, Viral
  • Operon
  • Promoter Regions, Genetic
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*

Substances

  • Viral Proteins
  • Endopeptidases
  • endolysin