Adaptive illumination based on direct wavefront sensing in a light-sheet fluorescence microscope

Opt Express. 2016 Oct 31;24(22):24896-24906. doi: 10.1364/OE.24.024896.


A methodology for the adaptive control and correction of phase aberrations in the illumination arm of a light-sheet fluorescence microscope has been developed. The method uses direct wavefront sensing on epi-fluorescent light to detect the aberration present in the sample. Using this signal, the aberrations in the illumination arm are subsequently corrected with a spatial light modulator in a feedforward mode. Adaptive correction, resulting in significant improvement in the axial resolution, has been demonstrated by imaging Tg(fli:GFP) zebrafish embryos.

MeSH terms

  • Animals
  • Embryo, Nonmammalian
  • Fluorescence
  • Microscopy, Fluorescence*
  • Optics and Photonics
  • Zebrafish