Minimally Invasive Liver Preconditioning for Hepatocyte Transplantation in Rats

Methods Mol Biol. 2017;1506:193-200. doi: 10.1007/978-1-4939-6506-9_13.

Abstract

In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.

Keywords: Hepatocyte isolation; Hepatocyte transplantation; Liver perfusion; Liver preconditioning; Portal vein embolization.

MeSH terms

  • Animals
  • Cell Separation / instrumentation
  • Cell Separation / methods
  • Cell Transplantation / instrumentation
  • Cell Transplantation / methods*
  • Gelatin / administration & dosage
  • Hepatocytes / transplantation*
  • Liver / blood supply
  • Liver / cytology
  • Liver / physiology*
  • Liver / surgery
  • Liver Regeneration*
  • Male
  • Minimally Invasive Surgical Procedures / methods
  • Perfusion / instrumentation
  • Perfusion / methods
  • Portal Vein / surgery*
  • Rats
  • Spleen / surgery
  • Transplantation Conditioning / methods*

Substances

  • Gelatin