Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes

Methods Mol Biol. 2017;1522:237-243. doi: 10.1007/978-1-4939-6591-5_18.

Abstract

Lipoplex preparations are heterogeneous mixtures of lipoplex particles of different structure. Because these structures determine the efficiency of genetic material delivery, it is important to characterize the distribution of particles of different types in lipoplex preparations with good statistics. We describe the application of flow fluorometry which allow producing such distributions (in terms of lipoplex particle size and composition) within minutes using basic flow cytometer (Pozharski and MacDonald, Anal Biochem 341:230-240, 2005).

Keywords: Cationic lipid; Flow cytometry; Heterogeneity; Lipoplex; Single particle detection; Size-stoichiometry distribution.

MeSH terms

  • Cations
  • DNA / chemistry*
  • Fluorometry / methods*
  • Lipids / chemistry*
  • Liposomes / chemistry
  • Staining and Labeling
  • Statistics as Topic

Substances

  • Cations
  • Lipids
  • Liposomes
  • DNA