Cryopreserved Adipose Tissue-Derived Stromal/Stem Cells: Potential for Applications in Clinic and Therapy

Adv Exp Med Biol. 2016;951:137-146. doi: 10.1007/978-3-319-45457-3_11.

Abstract

Adipose-Derived Stromal/Stem Cells (ASC) have considerable potential for regenerative medicine due to their abilities to proliferate, differentiate into multiple cell lineages, high cell yield, relative ease of acquisition, and almost no ethical concerns since they are derived from adult tissue. Storage of ASC by cryopreservation has been well described that maintains high cell yield and viability, stable immunophenotype, and robust differentiation potential post-thaw. This ability is crucial for banking research and for clinical therapeutic purposes that avoid the morbidity related to repetitive liposuction tissue harvests. ASC secrete various biomolecules such as cytokines which are reported to have immunomodulatory properties and therapeutic potential to reverse symptoms of multiple degenerative diseases/disorders. Nevertheless, safety regarding the use of these cells clinically is still under investigation. This chapter focuses on the different aspects of cryopreserved ASC and the methods to evaluate their functionality for future clinical use.

Keywords: Adipose tissue; Adipose-derived stromal/stem cells; Cell differentiation; Clinical trials; Cryopreservation; Human cells; Immunophenotype; Regenerative medicine.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adipocytes / cytology*
  • Adipocytes / physiology
  • Adipose Tissue / cytology*
  • Adipose Tissue / physiology
  • Adult
  • Biological Specimen Banks
  • Bone Regeneration
  • Cell Differentiation
  • Cell Proliferation
  • Cell Survival / drug effects
  • Cell- and Tissue-Based Therapy / methods*
  • Cryopreservation / methods*
  • Cryoprotective Agents / pharmacology
  • Dimethyl Sulfoxide / pharmacology
  • Ethylene Glycol / pharmacology
  • Humans
  • Mammaplasty / methods
  • Neurodegenerative Diseases / therapy
  • Stromal Cells / cytology*
  • Stromal Cells / physiology
  • Stromal Cells / transplantation

Substances

  • Cryoprotective Agents
  • Ethylene Glycol
  • Dimethyl Sulfoxide