Class I histone deacetylases regulate p53/NF-κB crosstalk in cancer cells

Cell Signal. 2017 Jan;29:218-225. doi: 10.1016/j.cellsig.2016.11.002. Epub 2016 Nov 9.

Abstract

The transcription factors NF-κB and p53 as well as their crosstalk determine the fate of tumor cells upon therapeutic interventions. Replicative stress and cytokines promote signaling cascades that lead to the co-regulation of p53 and NF-κB. Consequently, nuclear p53/NF-κB signaling complexes activate NF-κB-dependent survival genes. The 18 histone deacetylases (HDACs) are epigenetic modulators that fall into four classes (I-IV). Inhibitors of histone deacetylases (HDACi) become increasingly appreciated as anti-cancer agents. Based on their effects on p53 and NF-κB, we addressed whether clinically relevant HDACi affect the NF-κB/p53 crosstalk. The chemotherapeutics hydroxyurea, etoposide, and fludarabine halt cell cycle progression, induce DNA damage, and lead to DNA fragmentation. These agents co-induce p53 and NF-κB-dependent gene expression in cell lines from breast and colon cancer and in primary chronic lymphatic leukemia (CLL) cells. Using specific HDACi, we find that the class I subgroup of HDACs, but not the class IIb deacetylase HDAC6, are required for the hydroxyurea-induced crosstalk between p53 and NF-κB. HDACi decrease the basal and stress-induced expression of p53 and block NF-κB-regulated gene expression. We further show that class I HDACi induce senescence in pancreatic cancer cells with mutant p53.

Keywords: HDAC; HDACi; NF-κB; Replicative stress; Survivin; p53.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Cell Cycle Checkpoints / drug effects
  • Cell Line, Tumor
  • Cellular Senescence / drug effects
  • DNA Damage
  • DNA, Neoplasm / metabolism
  • Etoposide / pharmacology
  • Gene Expression Regulation, Neoplastic / drug effects
  • Histone Deacetylase Inhibitors / pharmacology
  • Histone Deacetylases / metabolism*
  • Humans
  • Hydroxyurea / pharmacology
  • Models, Biological
  • Mutation / genetics
  • NF-kappa B / metabolism*
  • Neoplasms / genetics
  • Neoplasms / metabolism*
  • Neoplasms / pathology*
  • Signal Transduction*
  • Tumor Suppressor Protein p53 / metabolism*
  • Vidarabine / analogs & derivatives
  • Vidarabine / pharmacology

Substances

  • DNA, Neoplasm
  • Histone Deacetylase Inhibitors
  • NF-kappa B
  • Tumor Suppressor Protein p53
  • Etoposide
  • Histone Deacetylases
  • Vidarabine
  • fludarabine
  • Hydroxyurea