Anti-fatigue activity of polysaccharide fractions from Lepidium meyenii Walp. (maca)

Int J Biol Macromol. 2017 Feb;95:1305-1311. doi: 10.1016/j.ijbiomac.2016.11.031. Epub 2016 Nov 10.

Abstract

The two fractions of polysaccharide MPS-1 and MPS-2 were extracted from Lepidium meyenii Walp. (maca) by water, and purified using a DEAE-52 and a Sephadex G-100 column. The molecular weight (MW) of MPS-1 was 7.6kDa, and the MW of MPS-2 was 6.7kDa. The MPS-1 was composed of xylose, arabinose, galactose and glucose, with the mole ratio 1:1.7:3.3:30.5; the MPS-2 was composed of arabinose, galactose and glucose, with the mole ratio 1:1.3:36.8. The IR spectrum implied that only α-pyranose existed in MPS-1, and both α-pyranose and β-pyranose existed in MPS-2. The anti-fatigue activities of MPS-1 and MPS-2 were measured by the forced swimming test, along with the determination of blood lactate (BLA), urea nitrogen (BUN), lactic dehydrogenase (LDH) activity and liver glycogen (LG). The results indicated that both MPS-1 and MPS-2 presented dose-dependently positive effects on the fatigue related parameters. Additionally, MPS-2 has a better anti-fatigue effect than MPS-1.

Keywords: Anti-fatigue; Maca (Lepidium meyenii Walp.); Polysaccharide.

MeSH terms

  • Animals
  • Arabinose / analysis
  • Blood Urea Nitrogen
  • Chemical Fractionation / methods
  • Dietary Carbohydrates / isolation & purification
  • Dietary Carbohydrates / pharmacology*
  • Dose-Response Relationship, Drug
  • Energy Metabolism / drug effects*
  • Fatigue / drug therapy*
  • Fatigue / metabolism
  • Fatigue / physiopathology
  • Galactose / analysis
  • Glucose / analysis
  • Glycogen / metabolism
  • Hydrolysis
  • L-Lactate Dehydrogenase / blood
  • Lactic Acid / blood
  • Lepidium / chemistry*
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Molecular Weight
  • Plant Extracts / chemistry
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Swimming
  • Xylose / analysis

Substances

  • Dietary Carbohydrates
  • Plant Extracts
  • Polysaccharides
  • Lactic Acid
  • Glycogen
  • Xylose
  • Arabinose
  • L-Lactate Dehydrogenase
  • Glucose
  • Galactose