Histamine synthesis by mouse T lymphocytes through induced histidine decarboxylase

Immunology. 1989 Feb;66(2):219-23.


When spleen cells of C57BL/6 mice or mast cell-deficient W/Wv mice were cultured, their histidine decarboxylase (HDC) activity increased with increases in the histamine concentration in the cells and the medium. Addition of concanavalin A (Con A) or Escherichia coli lipopolysaccharide (LPS) enhanced the increase. The removal of adherent cells reduced both the control HDC activity and the response to the mitogens. Purified T lymphocytes responded to Con A but not to LPS. Neither Con A nor LPS had any effect on B lymphocytes. Treatment of T cells with anti-Thy-1.2 and complement completely abrogated the induction of HDC. Histamine synthesis dependent on Con A by T cells was stimulated by the addition of conditioned medium from peritoneal adherent cells activated with LPS. The addition of recombinant interleukin-1 (rIL-1) or peritoneal adherent cells fixed with paraformaldehyde significantly enhanced HDC induction dependent on Con A in T cells. These results suggest that histamine is synthesized by T lymphocytes through HDC and that the reaction was enhanced by a soluble factor(s) released from macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carboxy-Lyases / metabolism*
  • Cells, Cultured
  • Concanavalin A
  • Histamine / biosynthesis*
  • Histidine Decarboxylase / metabolism*
  • Interleukin-1 / pharmacology
  • Lymphocyte Activation
  • Macrophages / physiology
  • Mice
  • Mice, Inbred Strains
  • Peritoneal Cavity / cytology
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / metabolism*


  • Interleukin-1
  • Concanavalin A
  • Histamine
  • Carboxy-Lyases
  • Histidine Decarboxylase