Direct Ethanol Production from Ionic Liquid-Pretreated Lignocellulosic Biomass by Cellulase-Displaying Yeasts

Appl Biochem Biotechnol. 2017 May;182(1):229-237. doi: 10.1007/s12010-016-2322-2. Epub 2016 Nov 14.

Abstract

Among the many types of lignocellulosic biomass pretreatment methods, the use of ionic liquids (ILs) is regarded as one of the most promising strategies. In this study, the effects of four kinds of ILs for pretreatment of lignocellulosic biomass such as bagasse, eucalyptus, and cedar were evaluated. In direct ethanol fermentation from biomass incorporated with ILs by cellulase-displaying yeast, 1-butyl-3-methylimidazolium acetate ([Bmim][OAc]) was the most effective IL. The ethanol production and yield from [Bmim][OAc]-pretreated bagasse reached 0.81 g/L and 73.4% of the theoretical yield after fermentation for 96 h. The results prove the initial concept, in which the direct fermentation from lignocellulosic biomass effectively promoted by the pretreatment with IL.

Keywords: Direct fermentation; Ethanol; Ionic liquid; Lignocellulosic biomass; Yeast.

MeSH terms

  • Aspergillus / drug effects
  • Aspergillus / enzymology
  • Biofuels*
  • Biomass
  • Cedrus / chemistry
  • Cellulases / metabolism
  • Cellulose / chemistry
  • Ethanol / metabolism*
  • Eucalyptus / chemistry
  • Fermentation
  • Fungal Proteins / metabolism
  • Glucose / biosynthesis*
  • Imidazoles / chemistry*
  • Imidazoles / pharmacology
  • Ionic Liquids / chemistry*
  • Ionic Liquids / pharmacology
  • Kinetics
  • Lignin / metabolism*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / enzymology
  • Trichoderma / drug effects
  • Trichoderma / enzymology
  • Xylose / biosynthesis*

Substances

  • 1-butyl-3-methylimidazolium
  • Biofuels
  • Fungal Proteins
  • Imidazoles
  • Ionic Liquids
  • lignocellulose
  • Ethanol
  • Cellulose
  • Lignin
  • bagasse
  • Xylose
  • Cellulases
  • Glucose