Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers

Methods Mol Biol. 2017;1486:483-509. doi: 10.1007/978-1-4939-6421-5_19.

Abstract

The myosin superfamily of molecular motors utilizes energy from ATP hydrolysis to generate force and motility along actin filaments in a diverse array of cellular processes. These motors are structurally, kinetically, and mechanically tuned to their specific molecular roles in the cell. Optical trapping techniques have played a central role in elucidating the mechanisms by which myosins generate force and in exposing the remarkable diversity of myosin functions. Here, we present thorough methods for measuring and analyzing interactions between actin and non-processive myosins using optical trapping techniques.

Keywords: Actomyosin; Kinetics; Mechanochemistry; Single molecule.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Actins / chemistry
  • Actins / metabolism
  • Kinetics
  • Molecular Motor Proteins / chemistry*
  • Molecular Motor Proteins / metabolism
  • Myosins / chemistry*
  • Myosins / metabolism
  • Optical Tweezers*
  • Protein Binding
  • Protein Interaction Domains and Motifs

Substances

  • Actins
  • Molecular Motor Proteins
  • Myosins