Protective effect of Silybum marianum and Taraxacum officinale extracts against oxidative kidney injuries induced by carbon tetrachloride in rats

Ren Fail. 2017 Nov;39(1):1-6. doi: 10.1080/0886022X.2016.1244070. Epub 2016 Nov 15.


The protective effect of the extracts of the plants Silybum marianum and Taraxacum officinale by carbon tetrachloride (CCl4) was researched. Sixty-six female Wistar albino rats were divided into six groups: Control, Silybum marianum, Taraxacum officinale, CCl4, Silybum marianum+ CCl4, Taraxacum officinale+CCl4. The Silybum marianum and Taraxacum officinale extracts were administered as 100 mg/kg/day by gavage. The CCl4 was administered as 1.5 mL/kg (i.p.). At the end of the trial period, in the serums obtained from the animals, in the CCl4 group it was found that the MDA level increased in the kidney tissue samples as well as in the ALP and GGT enzyme activities. It was also found that the GSH level and the GST enzyme activities decreased (p<.05). The microscopic evaluations showed that the CCl4 caused a serious hydropic degeneration, coagulation necrosis, and mono-nuclear cell infiltration in the kidney cell. In the animals where CCl4 and Silybum marianum and Taraxacum officinale extracts were applied together, it was found that the serum ALP and GGT enzyme activities decreased and that the MDA level decreased in the kidney tissue, and that the GSH level and GST enzyme activities increased. It was observed that the histopathological changes caused by the CCl4 toxicity were corrected by applying the extracts. Eventually, it was determined that the Silybum marianum was more effective. Silybum marianum and Taraxacum officinale extracts which were used against histopathological changes in the kidney caused by toxication showed a corrective effect, which were supported by biochemical parameters.

Keywords: Carbon tetrachloride; Silybum marianum; Taraxacum officinale; kidney; oxidative stress.

MeSH terms

  • Acute Kidney Injury / chemically induced
  • Acute Kidney Injury / drug therapy*
  • Animals
  • Antioxidants / pharmacology*
  • Carbon Tetrachloride / toxicity
  • Female
  • Kidney / drug effects
  • Kidney / pathology
  • Lipid Peroxidation / drug effects
  • Oxidative Stress / drug effects*
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Wistar
  • Silybum marianum / chemistry*
  • Taraxacum / chemistry*


  • Antioxidants
  • Plant Extracts
  • Carbon Tetrachloride