In Vitro Anti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

Mediators Inflamm. 2016;2016:3583684. doi: 10.1155/2016/3583684. Epub 2016 Oct 25.

Abstract

Trans-10-hydroxy-2-decenoic acid (10-H2DA), 10-hydroxydecanoic acid (10-HDAA), and sebacic acid (SEA) are the three major fatty acids in royal jelly (RJ). Previous studies have revealed several pharmacological activities of 10-H2DA and 10-HDAA, although the anti-inflammatory effects and underlying mechanisms by which SEA acts are poorly understood. In the present study, we evaluated and compared the in vitro anti-inflammatory effects of these RJ fatty acids in lipopolysaccharide-stimulated RAW 264.7 macrophages. The results showed that 10-H2DA, 10-HDAA, and SEA had potent, dose-dependent inhibitory effects on the release of the major inflammatory-mediators, nitric oxide, and interleukin-10, and only SEA decreased TNF-α production. Several key inflammatory genes have also been modulated by these RJ fatty acids, with 10-H2DA showing distinct modulating effects as compared to the other two FAs. Furthermore, we found that these three FAs regulated several proteins involved in MAPK and NF-κB signaling pathways. Taken together, these findings provide additional references for using RJ against inflammatory diseases.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry*
  • Cell Survival
  • Cytokines / metabolism
  • Decanoic Acids / chemistry*
  • Dicarboxylic Acids / chemistry*
  • Fatty Acids / chemistry*
  • Fatty Acids, Monounsaturated / chemistry*
  • Inflammation
  • Lipopolysaccharides
  • MAP Kinase Signaling System
  • Macrophages / cytology
  • Macrophages / metabolism
  • Mice
  • NF-kappa B / metabolism
  • Nitric Oxide / metabolism
  • RAW 264.7 Cells
  • Signal Transduction

Substances

  • 10-hydroxydecanoic acid
  • Anti-Inflammatory Agents
  • Cytokines
  • Decanoic Acids
  • Dicarboxylic Acids
  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • Lipopolysaccharides
  • NF-kappa B
  • Nitric Oxide
  • 10-hydroxy-2-decenoic acid
  • sebacic acid
  • royal jelly